anti mpo polyclonal rabbit igg conjugated to alexa fluor 350  (Bioss)

Journal: Biosensors

Article Title: NanoArrayPAD−X: Nanoprobe Array and 3D-µPAD for the Simultaneous Detection of Respiratory Pathogens and Biomarkers at the Point of Care

doi: 10.3390/bios15110715

Figure Lengend Snippet: Images of the NanoArrayPAD−5 and sample analysis workflow. ( A ) Device overview and components: sample pad with microfluidic system and fitting piece containing nanoprobe arrays. ( B ) Detection steps: ( i ) sample and blocking solution addition; ( ii ) nanoprobe transfer to detection areas; ( iii ) washing off excess nanoprobes; ( iv ) color intensity quantification to assess infection risk. PA: Pseudomonas aeruginosa ; KP: Klebsiella pneumoniae ; SA: Staphylococcus aureus ; EC: Escherichia coli ; MPO: myeloperoxidase.

Article Snippet: Rabbit anti-myeloperoxidase (MPO) polyclonal antibody was purchased from Bioss Antibodies (Woburn, MA, USA).

Techniques: Blocking Assay, Infection

Journal: Biosensors

Article Title: NanoArrayPAD−X: Nanoprobe Array and 3D-µPAD for the Simultaneous Detection of Respiratory Pathogens and Biomarkers at the Point of Care

doi: 10.3390/bios15110715

Figure Lengend Snippet: Nanoprobe optimization and cross-reactivity studies. Colorimetric signal for detecting PA ( A ) , KP ( B ), SA ( C ), and EC ( D ) at 0 (gray) and 10 7 CFU mL −1 (orange, blue, black, and purple), and MPO ( E ) at 0 (gray) and 3 μg mL −1 (pink) using nanoprobes modified with target-specific antibodies at different concentrations. Calibration plots for single targets at different concentrations (orange: PA; blue: KP; black: SA; purple: EC; pink: MPO; brown: S. maltophilia ; green: S. pneumoniae ; and red: S. epidermidis ) obtained with nanoprobes against PA ( F ), KP ( G ), SA ( H ), EC ( I ), and MPO ( J ). Error bars represent the SD of three independent experiments. S: colorimetric signal. PA: Pseudomonas aeruginosa ; KP: Klebsiella pneumoniae ; SA: Staphylococcus aureus ; EC: Escherichia coli ; MPO: myeloperoxidase. CFU: colony forming units. SD: standard deviation.

Article Snippet: Rabbit anti-myeloperoxidase (MPO) polyclonal antibody was purchased from Bioss Antibodies (Woburn, MA, USA).

Techniques: Modification, Standard Deviation

Journal: Biosensors

Article Title: NanoArrayPAD−X: Nanoprobe Array and 3D-µPAD for the Simultaneous Detection of Respiratory Pathogens and Biomarkers at the Point of Care

doi: 10.3390/bios15110715

Figure Lengend Snippet: Multiplexed detection with NanoArrayPAD−5. Signals from α−PA, α−KP, α−SA, α−EC, and α−MPO nanoprobes when analyzing samples containing PA ( A ), KP ( B ), SA ( C ), and EC ( D ) suspended in PBS and MPO in PBS ( E ), or spiked into a BAS sample ( F ). In ( A – D ), 0, 1, 3, 5, and 7 refer to 10 0 , 10 1 , 10 3 , 10 5 , and 10 7 CFU·mL −1 , respectively. Error bars represent the SD of three independent experiments. Dotted lines indicate the LOD, defined as blank + 3SD. S: colorimetric signal. PA: Pseudomonas aeruginosa ; KP: Klebsiella pneumoniae ; SA: Staphylococcus aureus ; EC: Escherichia coli ; MPO: myeloperoxidase. CFU: colony forming units. BAS: bronchial aspirate sample. AuNPs: gold nanoprobes. SD: standard deviation. LOD: limit of detection.

Article Snippet: Rabbit anti-myeloperoxidase (MPO) polyclonal antibody was purchased from Bioss Antibodies (Woburn, MA, USA).

Techniques: Standard Deviation

Journal: Biosensors

Article Title: NanoArrayPAD−X: Nanoprobe Array and 3D-µPAD for the Simultaneous Detection of Respiratory Pathogens and Biomarkers at the Point of Care

doi: 10.3390/bios15110715

Figure Lengend Snippet: NanoArrayPAD−5 detection of bacteria and biomarkers in liquefied BAS samples. Colorimetric signals from nanoprobes conjugated with antibodies against PA ( A ), KP ( B ), SA ( C ), EC ( D ), and MPO ( E ) were measured in 87 samples. Signal distributions for isolated target bacteria are shown in orange (PA), blue (KP), black (SA), and purple (EC), while controls (non-target pathogens, mixed flora, or other pathogens) are shown in green. Central bars represent means and dotted lines indicate the detection cut-off (mean + 2 SD). α-MPO signals ( E ) were higher in culture-positive samples (red). S: colorimetric signal. PA: Pseudomonas aeruginosa ; KP: Klebsiella pneumoniae ; SA: Staphylococcus aureus ; EC: Escherichia coli ; MPO: myeloperoxidase. CFU: colony forming units. BAS: bronchial aspirate sample. SD: standard deviation.

Article Snippet: Rabbit anti-myeloperoxidase (MPO) polyclonal antibody was purchased from Bioss Antibodies (Woburn, MA, USA).

Techniques: Bacteria, Isolation, Standard Deviation